C-flat holey carbon coated grids for cryo-TEM, thick
C-flat holey carbon coated grids for cryo-TEM, thick
Taxes and shipping applied at checkout.
C-flat is an ultra-flat, holey carbon-coated TEM support grid for transmission electron microscopy (TEM). Unlike competing holey carbon films, C-flat is manufactured without plastics, so it is clean upon arrival and the user has no residue to contend with.
C-flat, a product of patent-pending technology, offers an ultra-flat surface that improves particle dispersion and produces more consistent ice thickness. Deep-UV projection lithography is used for patterning, which guarantees the most precise and reliable hole sizes and shapes down to submicron features. The precise methods by which C-flat is manufactured eliminate artefacts such as excess carbon and edges around holes.
Applications
C-flat holey carbon grids provide the ideal specimen support to achieve high resolution data in cryo-TEM making them an ideal choice for single particle analysis, cryo electron tomography and automated TEM analysis.
Cryo-Electron Tomography (cryoET) and Single Particle Analysis ( SPA ):
Numerous researchers have reported that the ultra-flat surface of C-flat? leads to even ice thickness and uniform particle distribution within the hole areas. This optimal particle distribution results in superior data being collected as compared with other holey support films. 2um hole sizes are standard.
Automated TEM
C-flat? provides a regular array of analysis sites compatible with automated data collection software such as Leginon. This compatibility leads to more high-quality target sites per grid, along with more uniform ice thickness and particle distribution.
Product Line
C-flat is a holey carbon film supported by a standard TEM grid. C-flat products are fully specified by 4 parameters: the hole diameter and pitch of the holey carbon film and the material type and mesh size of the TEM grid. The following image illustrates these parameters
Standard Products
A variety of holey carbon film designs are required due to the cryoTEM's broad range of uses. And now that the product line has recently expanded, there is a C-flat holey carbon sheet available for almost any application. C-flatTM is the ideal holey carbon grid, whether 600 nm holes are required for extremely high magnifications with ultra-high resolution cameras or large open spaces are required for bigger specimens.
C-flat? is immediately available in several standard array patterns including hole diameters/hole spacings of 0.6/2, 1/1, 1/2, 1/4, 1.2/1.3, 2/1, 2/2, 2/4, 4/2. C-flat is supported by your choice of a 200 mesh or 400 mesh copper TEM grid and sold in quantities of 25,50 or 100.
Thick Products
C-Flat is available in a thick option that doubles the carbon thickness from approximately 20nm to 40nm. Thick C-flat product numbers end in -T, catalog numbers contain "CFT". Available in quantities of 50 and 100 per pack.