Colour Index #75290.
C16H14O6. F.W.302.29 CAS#5172-8-2
For use in Mayer's acid, Harris's alum haematoxylin, in Weigert's and Heidenhain's iron and other haematoxylins stains. Used in histology, cytology and also with plastic embedded tissues.
Chen, Chang S., J.Arch. of Path., 93,334 (1972).
Fax for 500g or 1kg price, we are most competetive.
C8H1804N2S F.W. 238.30 CAS #7365-45-9
Assay..................... > 99% pH Range................ 7.0-8.0 A(260) (1.0M in H20)...... < 0.05 Ash...................... < 0.1%
F.W. 161.40 B.P. 126.7°C. For fast preparation of soft insect tissues, also bacteria and some other "hardy" specimens in lieu of CPD.
Method: After complete dehydration up to 2 changes in abs. ethanol, treat specimens for 10min. in 1:1 ethanol : HMDS and then 3x10 minutes in pure HMDS. Dry specimen on filter paper in a glass Petri dish with lid ajar within a fume hood.
Slower drying by refrigerating the Petri dish overnight (and warm up before opening to avoid condensation) may be beneficial with sensitive specimens.Int.J.Insect.Morph.Embryol., 12(4),201 (1983); Stain Tech., 58(6),347 (1983)
For thin 'one micrometre' sections. By London Resin. Specially formulated for light microscopy. A low toxicity acrylic resin, it is easy to prepare and handle and is more adaptable for staining procedures when compared with the epoxy resins.
Benzoyl peroxide MSDS
- 500mL Histocryl resin
- 50g Benzoyl peroxide
- 10mL Accelerator
Colour Index #73015.
Shunway's stain for animal embryos. Used with acid fuchsin for staining Negri bodies. Stain Tech., 5,34 (1928) Counter-stain to haematoxylin for staining vaginal smears. Am.J.Anat., 61,505 (1937)
Anhydrous. Metal stain for nucleic acid. F.W. 221.18. J.Biophys.Biochem.Cytol.,11,257 (1961)
A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalysed monomer acts as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4. Features:
Thin sections (.5-2.0 micron) with excellent morphological structure preservation. Water-clear blocks; casts in 90 minutes max. at room temperature. Good lipid and enzyme retention when processing at low temperatures (4°C). Removal of JB-4 resin prior to staining is not necessary. Clearing agents such as xylene and chloroform are not needed. Higher clarity and contrast than with paraffin sections. Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue. All components may be stored at room temperature.
References: 1.Hofman E.O. & Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981) 2.Beckstead, J.H., Blood, 57(6):1008 (1981) 3.Brinn, N & Pickett, P., J. Histotech., 2(5):125-130 (1979) 4.Block, Matthew H., et al., Lab. Med., 13(5)(1982) 5.Helander, K.G., J. Microscopy, 132:223 (1983) 6.Cole, M.B., J. Microscopy, 127:139 (1982) 7.Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979) 8.Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980) 9.Horton, W.A., J. Histochem. Cytochem., 31:417 (1983) 10.Tacha, D.E. & Richard, T.C.., J. Histotech., 4(2):59 (1981)
JB-4 kit components:
A Romanowsky-type stain for peripheral blood smears.