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Note: Quorum Technologies have discontinued stand-alone glow discharge units. This feature is now included only in the KSC7620 and the KQ150 series as an optional accessory. We can supply a non-Quorum stand-alone glow discharge system.

The primary application of glow discharge is as a technique for the surface modification, or 'wetting', of newly-evaporated transmission electron microscopy (TEM) carbon support films.

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PELCO easiGlow™ Glow Discharge Cleaning System
PELCO easiGlow™ Glow Discharge Cleaning System
PELCO EASIGLOW™ GLOW DISCHARGE SYSTEM

Optimised for cleaning TEM grids

For one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored to facilitate consistent glow discharge results. Open the shipping box, plug it in, and you can start.

The PELCO easiGlow™ Glow Discharge Cleaning System is a compact, quick and easy to use standalone system. It is primarily designed for hydrophilisation of TEM carbon support films, which have the tendency to be hydrophobic. A glow discharge treatment with air will make a carbon film surface negatively charged (hydrophilic) which allows aqueous solution to spread easily.

With increasingly demanding imaging applications, using clean and consistent quality TEM grids and supports has become more important than ever. The PELCO easiGlow™ is an affordable and easy to operate glow discharge system for any TEM lab.

The practical chamber size with its newly designed glow discharge head and adjustable stage with glass slide holder allows for easy loading and fast turnaround times. The system is fully microprocessor controlled and includes an intelligent touch screen device for control and display of operation parameters. The PELCO easiGlow™ supports both hydrophilic and hydrophobic treatment with either negative or positive charge and includes two separately controlled gas inlets. The required vacuum level is set by an electronically controlled precision proportional valve. This novel approach eliminates manual setting with a needle valve. A gentle venting procedure ensures that the TEM grids are not disturbed when the system is vented.

Optional Accessories


Cat. No.DescriptionUnit
ETP91050Replacement Glass Chamber with sealed top plateeach
ETP91000‐2Replacement Touch Screen Styluseach
ETP91051O‐ring for Glow Discharge Chamberpk/2
ETP91053E91050 Protective L‐gasket, use with O‐ringeach
ETP91010‐16PELCO easiGlow™ Vacuum Connection Kit to KF16 Pump inleteach
ETP91010‐25PELCO easiGlow™ Vacuum Connection Kit to KF25 Pump inleteach
ETP91010‐30Dual Vacuum Connection Kit for Dual Chamber Glow Discharge System to KF 16 pump inleteach
ETP9609Exhaust filter for GD7 or #7010 Rotary Vacuum Pumpeach
ETP891‐35Ultragrade 19 Rotary Pump Oil for GD7, 1 ltreach

For more information and pricing for these accessories ‐ ask
PELCO easiGlow™ and GD7 Vacuum Systems are available in 110V, 60Hz ‐ ask

E91000
PELCO easiGlow™ glow discharge unit, 230V
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E91002
PELCO easiGlow™ dual chamber glow discharge unit, 230V
ea
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E91020
GD7 Vacuum system, 7m³/hr incl. connection kit, 230V, 50Hz
ea
QUOTE
E91022
GD7 Vacuum system, 7m³/hr incl. dual vacuum connection kit for E91002, 230V
ea
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ETP91050
Replacement Glass Chamber for E91000 with sealed top plate
AUD522.00
ea
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ETP91051
O-ring for Glow Discharge Chamber
AUD25.00
pk/2
BACKORDER 
ETP91053
Protective L-gasket, use with O-ring, for ETP91050
AUD136.00
ea
BACKORDER 
Lynx II Automated Tissue Processor for Histology and Microscopy
Lynx II Automated Tissue Processor for Histology and Microscopy
Reagent carousel
Reagent carousel
Lynx II Accessory
Lynx II Accessory
Lynx II Accessory
LYNX II AUTOMATED TISSUE PROCESSOR

Lynx II Automated Tissue Processor for Histology and Microscopy is a state-of-the-art tissue processor which is compatible with all plastic resins and paraffin waxes.

Introduction
Lynx II is designed to be the successor of the Lynx Tissue Processor with several enhancements including the optional processing of larger size samples for Histology.

The Lynx II holds 24 reagent vials for EM processing. Optional HP (Histology processing) may be done with 12 larger size reagent vials for HP processing. In both EM and HP modes, Lynx II has two, independently controlled, heating/cooling stations.

Operational Overview of Lynx II
Lynx II is a batch mode, tissue processing system. The tissues may be processed for EM or HP. Tissues of either type are processed in the same manner: Samples are mounted on the sample arm and reagents are loaded on the reagent carousel. Based on the selected program by the user, the Lynx II rotates the reagent carousel until the selected reagent station is at the processing station. At the processing station the sample is lowered in the reagent. Based on the selection of agitation and vacuum, the Lynx II periodically moves the sample arm to provide agitation and applies vacuum when the agitation is not in progress. Once the programmed time in a given reagent is over, the sample is raised and the next reagent is rotated in the processing station.

The operator may select the processing for EM or HP by the program numbers. Programs 1 through 10 are used for EM processing and programs 11-20 are used for HP processing. In addition to the proper program number selection, it is the responsibility of the end user to use the proper carousel positions and the appropriate type of reagent vials based on the selection of EM vs. HP processing. Once the unit is properly configured for EM or HP processing, the operator loads the reagent carousel and the samples, selects the desired program and starts the run.

At the end of the run, the sample is retained in the last reagent until the operator removes the sample.

Features

  • 12 reagent vials for HP processing, each 125mL volume
  • 24 reagent vials for EM processing, each 25mL volume
  • 10 Programs for EM and 10 programs for Histology
  • Two independently controlled heating/cooling stations
  • Easy replacement of reagent carousel
  • Paraffin processing
  • Sample agitation
  • Vacuum infiltration during processing
  • Choice of plastic and metal containers for improved chemical compatibility and heat transfer
  • Optional external media based interface to a PC for ease of programming and allowing for the editing of programs on a PC and the transfer of programs between the Lynx II and the PC
  • Built-in fume extraction and ability to connect the discharge hose to lab exhaust system
  • Internal battery back-up during power fail to protect the samples
  • Ability to continue run after power interruption
  • User Friendly Operator Interface
  • Optional external UPS for continued processing during longer power outages


Functional Specifications
System Configuration
1. Lynx II is designed with the following functional modules.

  • Rotary mechanism to rotate the reagents
  • EM and HP reagent carousel
  • Up/Down mechanism to move the sample in and out of the reagent as well as to provide the agitation
  • EM and HP Sample arms
  • Tight seal for all reagent containers except for the one at the processing station.
  • Vacuum pump and a pressure sensor or a switch
  • Two Peltier operated heating/cooling station with independent temperature sensors and controls
  • Fume extraction fan and air flow sensor


2. Reagent Carousel
A reagent carousel may be installed, either a 24-position carousel for holding EM reagents or a 12-position carousel for holding HP reagents. The EM carousel holds cylindrical vials that are approx. 1.2" in diameter and 2.5" tall whereas HP vials are approx. 2.2" diameter (to hold 1.75" x 1.2" cassette flat) and 2.5" tall. The carousel is approx. 12" in diameter. Each vial has a flange for ease of handling. The vials are available in either plastic or aluminium. The vials may be mixed and matched between the metal and the plastic vials on a given carousel for certain kinds of processing.

The Lynx II has a built in sensor to detect that the proper reagent vial types (EM vs. HP) are installed on the unit before starting a run. Ability to detect only one of the two vial types is sufficient.

3. Sample Arm
The sample arm holds either an EM sample holder assembly or an HP sample holder assembly. Each sample holder assembly is designed to mount on the arm at a location to centre the samples in its respective reagent container. The Sample Arm top may be changed to accommodate different samples.

4. Agitation and Vacuum
The arm provides a gentle agitation (approx. 5 mm up and down travel at a rate of 2.5 mm per second or one up/down agitation per second) once the sample is in the reagent container. Five agitation strokes are executed in 5 seconds and repeated every 45 seconds. If vacuum infiltration is selected, the agitation will be applied for 5 seconds followed by 40 seconds of vacuum. These agitation-only or the agitation-vacuum cycles will be repeated for the duration of the station time.

The sample holder assembly has a fitting to attach the vacuum hose from the vacuum pump.
5. Heating-Cooling Stations
The Lynx II provides 2 independent, identical heating-cooling stations (HCS). Each HCS is heated or cooled by a Peltier module and has its own temperature sensor. One HCS is located at the sample processing station and the second one is located one position prior to the sample processing station. This arrangement allows for the pre-heating or pre-cooling of the reagent for a program where the reagents are arranged in a sequential, contiguous manner.

Each HCS can be programmed to be between 4 deg. C and 65 deg. C. The accuracy of the actual temperature will be ± 2 deg. C measured on the surface of the Heating – Cooling station. The set point at the HCS surface is reached within 7 minutes of the arrival of the reagent at the station.

6. Fume Control
Lynx II provides a fume extraction fan to direct fumes from the reagent/ sample area to an exhaust port. An external hose may be connected to the unit and the hose may be directed to a fume hood or to laboratory exhaust system. The fume extraction is designed to ensure that the fumes are diverted away from electronics and the mechanism components.

7. Lid/Cover
An acrylic cover with a latch is part of the fume control system. Controller of the system can detect if the cover is latched or not. By default, a run cannot be started (and will pause) if the cover is not in place. However,there is a software override will allow for the running of the unitt without closing the cover.

8. Seal for the Reagent Vials
A tight seal is provided to cover all reagent vials except for the one under the processing station.

Reagents Used
Materials for various components and subsystems of Lynx II are compatible with the following reagents during the processing.

HP Processing

  • Formalin
  • Alcohol
  • Xylene
  • Xylene Substitutes
  • Water
  • Paraffin
  • 10% bleach solution (to wipe clean the system)


EM Processing

  • Buffers
  • Osmium Tetroxide
  • Ethyl Alcohol
  • Acetone
  • Propylene Oxide
  • Epoxy/acrylic resins


9. Operation During Power Outage
The Lynx II contains a small internal battery (that is capable of moving the sample from up position to down position and to move the carousel in the next reagent position at least three times without being re-charged) to preserve the sample during a power outage. When the electronics in Lynx II detects that the A/C power is about to fail, the Lynx II will shut down the power to the Peltier elements at both heating/cooling stations. Next, it will ensure that the samples are down in a reagent container. If the samples are out of a reagent vial (since Lynx II was just about to rotate the carousel to the next reagent position or was in the middle of the rotation), the carousel will be rotated by one position and then the samples will be lowered in to the reagent at the processing station. The time when the power fail warning was activated will be recorded and the unit will go in the stand by mode.

Once the power is restored and is maintained for at least 30 seconds, Lynx II will resume the run. As Lynx II resumes run, the station time count down does not resume until the heating/cooling stations have reached the desired temperatures. The station time countdown does not begin for additional 60 minutes if the temperature at the processing station or at the previous station was above 55 deg. C during an HP run. This feature ensures that the paraffin at either of the two stations completely melts before moving to the next station

Specifications:

Dimensions:
Max Width:22.5"
Max Depth:19.0"
Max Height:12.0" (cover closed)
Max Weight50lb (32 kg)
Electrical Design:
Display:8 lines x 20 column text only LCD
Keypad:Membrane style keypad
with tactile feed back
Mechanism Drive:Stepper Motors
Power Requirements:100 - 115 volts AC, 50-60 Hz, 8 Amps Max

230 volts AC, 50-60 Hz, 4 Amps Max
Back-up power:NiCd Battery, 12 volts, 1 Amp-hour
Fuse Rating:
100 – 115 volt version8 Amps x 250 Volts AC,
Time Delayed (Slo-Blo)
230 volt version5 Amps x 250 Volts AC, Time Delayed (Slo-Blo)
Operating Environmental Conditions:
Ambient Temperature:20 to 32 o C (70 to 90° F)
Relative Humidity:20-80% non-condensing
Ambient Pressure:28"-32" (70-80 mm) of Hg
Storage/Transportation Conditions:
Temperature:10-40 deg C (50 to 102° F)
Humidity:15%-80% non-condensing
Pressure:28"-32" (70-80 mm) of Hg

Lynx II accessories and spare parts
L12610 Histology Kit
(Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers (36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses.

L12612 Electron Microscopy Kit
(EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers (72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses.

L12655 EM Reagent Vials, Bag of 72
L12656 EM Regent Vial Cap, Bag of 72
L12657 HP Reagent Vials, Bag of 36
L12658 HP Reagent Vial Cap, Bag of 36
L12659 EM Aluminium Container (Box of 24)
L12660 HP Aluminium Container (Box of 12)
L13154 EM Sample Holder Assembly (Basket Stem Assembly)
L12663 HP Cassette Holder Assembly
L12661 Vacuum Pump Tubing (1 each)
L12662 Vial Seal Assembly
L12995 EM Sample Arm Seal
L12996 HP Sample Arm Seal
L12952 EM Carousel (Without Aluminium Containers)
L12953 HP Carousel (Without Aluminium Containers)
L12664 Replacement Back-up Battery
L12665 Lynx II Operator Manual
L12667 Lynx II Service Manual

The Lynx II for Microscopy comes with: EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers(72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses.

The Lynx II for Histology comes with: Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers(36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses.

The Lynx II for Microscopy and Histology comes with everything found in both units.

All of the above accessories may be purchased separate or in the kit.

Consumables for Lynx I and II are on page H3

EL12600
Lynx II automated tissue processor, for microscopy
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EL12601
Lynx II automated tissue processor, for histology
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EL12602
Lynx II automated tissue processor, for microscopy & histology
ea
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Vial seal assembly
E300-9016
USER REPLACEABLE PARTS FOR LYNX I

Lynx 1 has not been produced for many years. Parts are supplied from a stock but these will exhaust at some point. Please consider initiating the purchase of Lynx 2. A trade in of AUD500 is given when purchase of a Lynx 2 system.

E300-0008
Location pin for LYNX processor
AUD61.00
ea
BACKORDER 
E300-0015
Cap (TurnTable Lockings) for LYNX processor
AUD122.00
ea
BACKORDER 
E300-0139
User manual for LYNX processor
AUD70.00
ea
BACKORDER 
E300-8041
Exhaust Hose for LYNX processor
AUD98.00
3m
BACKORDER 
E300-8105
Printer data cable for LYNX processor
AUD343.00
ea
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E300-9010
Basket stem and foot assembly for LYNX processor
AUD147.00
ea
BACKORDER 
E300-9011
Loading jig for LYNX processor
AUD260.00
ea
ADD TO CART 
E300-9014
Heater/Cooler for LYNX processor
ea
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E300-9016
Vial seal assembly for LYNX processor
AUD776.00
ea
BACKORDER 
E300-9017
Vial lid assembly for LYNX processor
AUD96.00
ea
BACKORDER 
E300-9023
Vial retaining band for LYNX processor
AUD87.00
ea
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E300-9100
Starter kit for LYNX processor
AUD905.00
ea
BACKORDER 
E300-9500
Assembly Heater/Cooler for LYNX tissue processor
ea
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E306-0830-0001
Key for LYNX processor
AUD19.00
ea
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