NIGHTSEAâ¢ Stereo Microscope Fluorescence Adapter
Adapt your existing lab stereo microscopes for fluorescence
The Nightsea™ stereo microscope fluorescence adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.
This simple system is excellent for:
- Quick screening of your fluorescent genotypes -Drosophila, zebrafish, C. elegans,...
- Genotype sorting
- Fluorescence-aided dissection, injection, or micromanipulation
- Freeing up your research-grade fluorescence microscopes for more demanding work
- New faculty start-up budgets
- Bringing fluorescence into the teaching laboratory
Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel
Fluorescence isn't just for research microscopes anymore
- Now sort on your laboratory-level stereos
- Use to facilitate micromanipulation and dissection
- Expand from your research lab to your classroom
- Installs in seconds - just clicks into place
- Interchangeable excitation/emission combinations
- Move from microscope to microscope
- No modification to your micoscope needed
Economical - more glow for the dough
- Stretch your lab budget
- Inexpensive enough for classroom use
Grows as your lab grows
- Buy just what you need now (up to 5 different wavelength sets)
- Add more as your needs expand
The stereo microscope fluorescence adapter system consists of
- Flexible gooseneck lamp base with power supply
- Light head
- Ring adapter for microscope
- Barrier filter
- Filter shield
The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light filter combinations.
The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes.
Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
|RB - Royal Blue||440-460nm||500nm LP||GFP, eGFP, fluorescein...|
|RB-GO||440-460nm||500-560nm BP||GFP, eGFP, fluorescein...|
|CY - Cyan||490-515nm||550nm LP||YFP, Venus, Lucifer Yellow...|
|GR - Green||510-540nm||600nm LP||DsRed, dTomato...|
|VI - Violet||400-415nm||460nm LP||CFP, ...|
|UV - Ultra Violet||360-380nm||415nm LP||DAPI, ...|
Microscope Mounting Adapter - fits up to 67mm standard.
Green-only barrier filter
The green-only barrier filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this new filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.
The Eclipse MicroTent™ is a unique product for fluorescence microscopy that provides local darkness around conventional laboratory stereo microscopes. Fluorescence microscopes are kept in dark rooms for good reason - fluorescence can be weak and in many cases it can be difficult to see well if there is any ambient light. Microscopes may be on lab benches in shared spaces, near windows, or in other difficult-to-darken locations such as in the field. Turning off overhead lights can help but inconveniences others.
The Eclipse Microtent creates local darkness around a microscope while still providing easy access to the sample stage and the focus and zoom controls. It is designed for stereo microscopes but could potentially be used with many varieties of compound microscopes.
- Opening for the microscope oculars with elastic sleeve to minimize light entry
- Large front flap provides easy access to the sample stage and can fasten open
- Arm slots on sides to provide access to focus and zoom controls
- User-customizable feedthrough patches to provide additional penetrations for camera port, power cords, CO? lines, or other features as you need
- Tru-Block™ Eye Shields included with every Eclipse MicroTent
- Folds flat for storage
- Dimensions: 460 x 300 x 500mm
Light entering your eyes from the side can interfere with what you want to see in microscopy in general, and fluorescence microscopy in particular. Eye cups are available, but the standard ones don't extend far from the microscope and don't do a good job as ambient light increases. Our soft, molded rubber high-sided microscope eye shields are the answer. The tall wings extend up far enough to truly shield your eyes from any level of ambient light and eliminate distractions so that you can see your subject better. Two pairs (one Standard, one Compact) will be included with every Eclipse MicroTent™, and you can also purchase them separately.
The Tru-Block Eye Shields are available in two sizes:
- Standard - fits 36 - 45mm
- Compact - fits 28 - 37mm
Each addâon excitation/emission set consists of:
- Light Head*
- Barrier Filter
- Viewing Shield
- Padded Storage Box
*Please note: Light Head connectors come in two types. They are not interchangeable. The new style connector is called a BNC, and the original connector is a V1. There are two differences â the connector on the end of the gooseneck and the mating connector on the back of the light head. It is easy to tell them apart:
- The BNC connector on the gooseneck is silver, while the V1 connector is black.
- The BNC light heads have the letters BNC printed on the side.
A white light head is also available as an illumination option for the Stereo Microscope Fluorescence Adapter. Now if you are using the NIGHTSEA system for fluorescence you do not need a separate light source for your white-light work - just exchange the fluorescence excitation light head module for the white-light module in a matter of seconds. Extremely convenient for general illumination and as a focusing aid for fluorescence imaging.
Rapid screening of your fluorescent transgenic experiments
The Model DFP Dual Fluorescent Protein Flashlights include two different, switch-selectable high intensity LEDs for excitation of fluorescent proteins. For years the most popular fluorescent colors have been green (GFP, eGFP, fluorescein, etc.) and red (DsRed, TdTomato, etc.), and we offered only one version of the DFP, with a combination of blue and green LEDs for exciting these fluorophores. This is still our 'standard' DFP product, but we are now offering build-to-order combinations using any two of the excitation colors that are available with our popular Model SFA Stereo Microscope Fluorescence Adapter.
With every DFP light you will receive two pairs of barrier filter glasses and a convenient storage case. The glasses are well matched to the excitation so that they block the reflected excitation light while transmitting the fluorescence with high efficiency, providing excellent viewing contrast.
|RB - Royal Blue||440-460nm||500nm LP||GFP, fluorescein, lucifer yellow, ...|
|CY - Cyan||490-515nm||550nm LP||YFP, Venus, ...|
|GR - Green||510-540nm||600nm LP||DsRed, TdTomato, RFP, ...|
|VI - Violet||400-415nm||460nm LP||BFP, CFP, ...|
|UV - Ultra Violet||360-380nm||415nm LP||DAPI, ...|
|Dimensions||5 x 3 x 2|
|Battery Type||4 C-cell|
|Bulb Type||Two high intensity 3W LED|
|Burn Time||4 hours at full power|
|Lamp Life||10,000 hours|
The BlueStar flashlight combined with the matched barrier filter glasses is a convenient, powerful tool for exciting fluorescence in many subjects.
The BlueStar uses the latest in high intensity LED technology combined with specialized optics to produce an ultra-tight, ultra-bright 10-degree beam pattern for maximum excitation of fluorescence.
Comes with adjustable, cushioned wrist lanyard, rubber glare guard. Made in USA.
|Bulb Type||High intensity 1W LED Royal Blue - 440 - 460nm|
|Burn Time||6 hours at full power|
|Lamp Life||>10,000 hours|
Styles 1 and 2 fit over eyeglasses, Style 3 does not.
Glasses meet ANSI Z87.1 impact standards for safety glasses.