DiATOME DIAMOND KNIVES
ProSciTech is an agent for DiATOME. We supply these top quality diamond knives at excellent prices. Why purchase a second-rating knife and hassle with a greater chance of a warranty return, more waiting periods and a lower service life of an inferior knife? Send an email and ask for price and availability. Price includes freight into your store. We accept purchase orders from Universities and other large organisations or credit card purchases.

Unique characteristics
  • DiATOME offers incomparable durability of the diamond edge. DiATOME knives have been known to last for many years without resharpening.
  • All DiATOME diamonds are of the highest possible quality with a regular crystal structure. The crystal orientation of the diamond permits a final polishing parallel to the cutting edge, which gives a perfect nick free edge.
  • Uniform sections are easily reproducible.
  • DiATOME knives are guaranteed for an unlimited number of resharpenings.
  • When we resharpen our diamond knives, we restore them to their original condition. Your resharpened DiATOME diamond knife will have the same length, the identical cutting edge and carry the same guarantee of quality as the day it first left our factory.
  • Our diamonds are fastened into a metal shank which is securely screwed into the boat and then sealed. The sealing material is hydrophilic, allowing for easy wetting of the cutting edge, and has no effect on the stability of the knife.
  • For our cryo knives we use a special epoxy resin for sealing which is chemical and temperature resistant.
  • Before delivery each knife, new or resharpened, is subjected to extensive testing for its ability to cut accurately without scoring or compression. Only if its performance passes our tests will we ship it to you.
  • The DiATOME boat is designed in such a manner that the water surface is horizontal when the clearance angle is set. This results in stationary water surface and good reflection.
  • DiATOME's universal knife boats are designed to fit all brands of ultramicrotomes without the need for an adapter.
  • The shape of the DiATOME boat offers the advantage of easy pick-up of the floating sections.
Knife
Angle
Sizes
Cutting Range
Application
Ultra
35°
1.5-4.0mm
30-150 nm
Standard applications
 
 
1.5-4.0mm
10-30 nm
Electron Spectroscopic Imaging (ESI), 3D Reconstruction, etc.
 
 
1.5-4.0mm
50nm - 1µ
Cryo sectioning
 
 
1.5-4.0mm
100nm-2µm
Alternating ultra-thin/semi-thin sectioning
Ultra
45°
1.5-4.0mm
30-200 nm
Standard applications
 
 
1.5-4.0mm
50nm-1µ
Alternating ultra-thin/semi-thin sectioning
Ultra Jumbo
35°
2.0, 3.0mm
 
For longer section ribbons
 
45°
2.0, 3.0mm
 
For longer section ribbons
Ultra AFM
35°
2.0, 3.0mm
10-100nm
Surface sectioning for biological and material
specimens for AFM investigation
Ultra Sonic
35°
3.0mm
10nm-80nm
Ultra thin sections without compression
Cryo Dry
25°
3.0mm
25nm-200nm
Frozen hydrated specimens
Cryo Immuno
35°
2.0, 3.0mm
30nm-1µm
Cryo-immuno-chemistry
Cryo AFM
35°
2.0, 3.0mm
10-100nm
Surface sectioning for biological and material
specimens for AFM investigation
Cryo Dry
35°
1.5-4.0mm
30nm-1µ
Frozen biological and material samples such as polymers, rubbers and paints
 
45°
1.5-4.0mm
30nm-1µ
Routine cryo sectioning
Cryo Wet
35°
1.5-4.0mm
30nm-1µ
Frozen biological and material samples such as polymers, rubbers and paints
 
45°
1.5-4.0mm
30nm-1µ
Routine cryo sectioning
Trimtool 20
20°
 
 
Trimming of sucrose protected as well as frozen hydrated biological samples and polymers
Trimming of epoxy and acrylic embeddings as
well as polymers and non-ferrous metals
Trimtool 45
45°
 
 
Trimming of sucrose protected as well as frozen hydrated biological samples and polymers
Trimming of epoxy and acrylic embeddings as
well as polymers and non-ferrous metals
Histo
45°
4.0, 6.0, 8.0mm
0.2-5µm
Sectioning of biological and material specimens non embedded or embedded (plastic or epoxy) for light microscopy
Histo Cryo Wet
45°
4.0, 6.0mm
0.2-5µm
Cryo sectioning with a boat for light microscopy
Histo Cryo Dry
45°
4.0, 6.0mm
0.2-5µm
Cryo Sectioning with a triangular holder for light microscopy
Histo Jumbo
45°
6.0, 8.0mm
0.2-5µm
Immuno-histo-chemistry and 3D reconstruction
Static Line II
 
 
 
Reduction of static charging.

Guarantee
Before delivery, each knife, new or resharpened, is subject to extensive testing. Perfect sectioning (in the respective thickness range) over the entire cutting edge is guaranteed. The knives are guaranteed for an unlimited number of resharpenings.

Resharpened diamond knives
Any DiATOME knife can be resharpened an unlimited number of times. Resharpened DiATOME knives undergo the same stringent optical checking and sectioning tests as new knives. A diamond knife resharpened by DiATOME is the same high quality as a new one.
Your resharpened DiATOME knife will have the same length, the identical cutting edge and carry the same guarantee of quality as the day it first left the factory.
Damaged or discoloured boats will be replaced at no charge.
Note for overseas customers: If you send us a diamond knife for resharpening, you must make the Custom's Declaration: No commercial value. Returning for repair. Otherwise, you will be charged the duty payable on the knife.

Resharpened diamond knife with exchange
Rather than having your own knife resharpened, the knife can be exchanged for an already resharpened knife. In the exchange service a type change is also possible (ultra to cryo, 45° to 35°, small knives to large ones, etc). This includes changing the boat to your desired style.

Custom diamond knives and boats
Upon request, we can supply different coloured (black) and shaped boats (Huxley for instance) at no extra cost. Certain applications require special knives and/or tools, outside the standard knives and tools available. For years DiATOME has been manufacturing custom diamond blades, tools and knives for customers, meeting all of their specific needs. There is no charge for this service, only the regular cost of the knife once you have approved it.

Delivery time and shipping
All new knives as well as exchange knives are available for delivery in 2 weeks. For our resharpening service, the turn-around time is no longer than 6 weeks from date that the knife is received by us. All of our knives, new or resharpened, are shipped at our expense.

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DiATOME Diamond Knives
ASK
Ultra Diamond Knives
Ultra Diamond Knives
THE ROOM TEMPERATURE ULTRA-THIN COLLECTION

The Ultra knives are available with 35 and 45 degree angles. The optimal thickness range for cutting with these knives is between 30 - 150nm. Sizes range from 1.5 - 4.0mm as a standard. Other sizes are available upon request. All of the knives are tested within a thickness range of 30 - 150nm. Whether your needs are biological or materials related, in EM or LM, we have a knife to suit your needs.

Ultra 45°
Recognised as the knife for routine sectioning. It is a good compromise between section quality and durability.
The 45° knife is recommended for the sectioning of extremely hard and brittle materials such as ceramics. In this domain it has replaced the formerly recommended 55° knife.

Ultra 35°
The capabilities of the 35° diamond knives have been shown in two publications by J.Jésior (Ref 3, 4). He proved markedly reduced compression, smoother section surfaces and better structural preservation with the use of our 35° diamond knives.
In the meantime a great number of scientists have noticed and recognised the advantages of the 35° knives for the sectioning of Lowicryls, inhomogenous samples such as undecalcified bone, dental materials, etc.
The 35° knives have proved advantageous in the sectioning of soft industrial samples such as metals and polymers, as well as for hard and brittle samples, for example semiconductors (Si, GaAs, etc), superconducting oxides, nanocrystalline ceramics.
The use of 35° knives leads to reduced compression in soft samples and to less breaking in hard and brittle samples.

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Ultra-thin Diamond Knives
ASK
DiATOME ULTRA DIAMOND KNIVES, 35°
UU35-15
Diamond knife, Ultra, 35°, 1.5 - 1.7mm, new
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QUOTE
UU35-15E
Diamond knife, Ultra, 35°, 1.5 - 1.7mm, exchanged
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QUOTE
UU35-15R
Diamond knife, Ultra, 35°, 1.5 - 1.7mm, resharpened
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QUOTE
UU35-18
Diamond knife, Ultra, 35°, 1.8 - 2.0mm, new
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QUOTE
UU35-18E
Diamond knife, Ultra, 35°, 1.8 - 2.0mm, exchanged
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QUOTE
UU35-18R
Diamond knife, Ultra, 35°, 1.8 - 2.0mm, resharpened
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QUOTE
UU35-21
Diamond knife, Ultra, 35°, 2.1 - 2.3mm, new
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QUOTE
UU35-21E
Diamond knife, Ultra, 35°, 2.1 - 2.3mm, exchanged
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QUOTE
UU35-21R
Diamond knife, Ultra, 35°, 2.1 - 2.3mm, resharpened
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QUOTE
UU35-24
Diamond knife, Ultra, 35°, 2.4 - 2.6mm, new
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QUOTE
UU35-24E
Diamond knife, Ultra, 35°, 2.4 - 2.6mm, exchanged
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QUOTE
UU35-24R
Diamond knife, Ultra, 35°, 2.4 - 2.6mm, resharpened
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QUOTE
UU35-25
Diamond knife, Ultra, 35°, 2.5 - 2.7mm, new
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QUOTE
UU35-25E
Diamond knife, Ultra, 35°, 2.5 - 2.7mm, exchanged
ea
QUOTE
UU35-25R
Diamond knife, Ultra, 35°, 2.5 - 2.7mm, resharpened
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QUOTE
UU35-30
Diamond knife, Ultra, 35°, 3.0 - 3.4mm, new
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QUOTE
UU35-30E
Diamond knife, Ultra, 35°, 3.0 - 3.4mm, exchanged
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QUOTE
UU35-30R
Diamond knife, Ultra, 35°, 3.0 - 3.4mm, resharpened
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QUOTE
UU35-35
Diamond knife, Ultra, 35°, 3.5 - 3.9mm, new
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QUOTE
UU35-35E
Diamond knife, Ultra, 35°, 3.5 - 3.9mm, exchanged
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QUOTE
UU35-35R
Diamond knife, Ultra, 35°, 3.5 - 3.9mm, resharpened
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QUOTE
UU35-40
Diamond knife, Ultra, 35°, 4.0 - 4.4mm, new
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QUOTE
UU35-40E
Diamond knife, Ultra, 35°, 4.0 - 4.4mm, exchanged
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QUOTE
UU35-40R
Diamond knife, Ultra, 35°, 4.0 - 4.4mm, resharpened
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QUOTE
DiATOME ULTRA DIAMOND KNIVES, 45°
UU45-15
Diamond knife, Ultra, 45°, 1.5 - 1.7mm, new
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QUOTE
UU45-15E
Diamond knife, Ultra, 45°, 1.5 - 1.7mm, exchanged
ea
QUOTE
UU45-15R
Diamond knife, Ultra, 45°, 1.5 - 1.7mm, resharpened
ea
QUOTE
UU45-18
Diamond knife, Ultra, 45°, 1.8 - 2.0mm, new
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QUOTE
UU45-18E
Diamond knife, Ultra, 45°, 1.8 - 2.0mm, exchanged
ea
QUOTE
UU45-18R
Diamond knife, Ultra, 45°, 1.8 - 2.0mm, resharpened
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QUOTE
UU45-21
Diamond knife, Ultra, 45°, 2.1 - 2.3mm, new
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QUOTE
UU45-21E
Diamond knife, Ultra, 45°, 2.1 - 2.3mm, exchanged
ea
QUOTE
UU45-21R
Diamond knife, Ultra, 45°, 2.1 - 2.3mm, resharpened
ea
QUOTE
UU45-24
Diamond knife, Ultra, 45°, 2.4 - 2.6mm, new
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QUOTE
UU45-24E
Diamond knife, Ultra, 45°, 2.4 - 2.6mm, exchanged
ea
QUOTE
UU45-24R
Diamond knife, Ultra, 45°, 2.4 - 2.6mm, resharpened
ea
QUOTE
UU45-25
Diamond knife, Ultra, 45°, 2.5 - 2.7mm, new
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QUOTE
UU45-25E
Diamond knife, Ultra, 45°, 2.5 - 2.7mm, exchanged
ea
QUOTE
UU45-25R
Diamond knife, Ultra, 45°, 2.5 - 2.7mm, resharpened
ea
QUOTE
UU45-30
Diamond knife, Ultra, 45°, 3.0 - 3.4mm, new
ea
QUOTE
UU45-30E
Diamond knife, Ultra, 45°, 3.0 - 3.4mm, exchanged
ea
QUOTE
UU45-30R
Diamond knife, Ultra, 45°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UU45-35
Diamond knife, Ultra, 45°, 3.5 - 3.9mm, new
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QUOTE
UU45-35E
Diamond knife, Ultra, 45°, 3.5 - 3.9mm, exchanged
ea
QUOTE
UU45-35R
Diamond knife, Ultra, 45°, 3.5 - 3.9mm, resharpened
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QUOTE
UU45-40
Diamond knife, Ultra, 45°, 4.0 - 4.4mm, new
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QUOTE
UU45-40E
Diamond knife, Ultra, 45°, 4.0 - 4.4mm, exchanged
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QUOTE
UU45-40R
Diamond knife, Ultra, 45°, 4.0 - 4.4mm, resharpened
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QUOTE
Ultra Jumbo Diamond knife
Ultra Jumbo Diamond knife
DiATOME ULTRA JUMBO DIAMOND KNIVES

The Ultra diamond knife is now available in Jumbo with 35° and 45° angles and in 2.0mm and 3.0mm sizes. The larger boat accommodates long ribbons for serial sections or when searching for a particular structure.

UUJ35-20
Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, new
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QUOTE
UUJ35-20R
Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, resharpened
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QUOTE
UUJ35-30
Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, new
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QUOTE
UUJ35-30R
Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UUJ45-20
Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, new
ea
QUOTE
UUJ45-20R
Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, resharpened
ea
QUOTE
UUJ45-30
Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, new
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QUOTE
UUJ45-30R
Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, resharpened
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QUOTE
Cryo Diamond Knives
Cryo Diamond Knives
LOW TEMPERATURE ULTRA-THIN COLLECTION
Cryo sectioning for electron microscopy is performed in cryo-ultramicrotomes, generally at -100°C, which prevents ice crystal formation. Prior to sectioning, biological specimens are vitrified by most rapid freezing to about -160°C. Cryo diamond knives are indispensible tools in this technically complex procedure.
Available for either wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1µm.
Cutting edge sizes 1.5 - 4mm.

DiATOME Cryo diamond knives may be used for all low temperature situations, such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.
There are numerous advantages to our Cryo diamond knives compared with glass knives:
  • They produce thinner sections
  • Ultrathin and semithin sections with the same knife
  • Easy to produce long ribbons of sections
  • Less compression and greater uniformity of sections
  • Time saving. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.
  • Diamond is the best temperature conductor - better than gold. No waiting for temperature equilibration


Cryo 25°
The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.

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DiATOME Cryo Knives
ASK
DiATOME CRYO DRY DIAMOND KNIVES, 25° and 35°
UCD25-30
Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, new
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QUOTE
UCD25-30R
Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UCD35-15
Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, new
ea
QUOTE
UCD35-15E
Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, exchanged
ea
QUOTE
UCD35-15R
Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, resharpened
ea
QUOTE
UCD35-18
Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, new
ea
QUOTE
UCD35-18E
Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, exchanged
ea
QUOTE
UCD35-18R
Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, resharpened
ea
QUOTE
UCD35-21
Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, new
ea
QUOTE
UCD35-21E
Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, exchanged
ea
QUOTE
UCD35-21R
Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, resharpened
ea
QUOTE
UCD35-24
Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, new
ea
QUOTE
UCD35-24E
Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, exchanged
ea
QUOTE
UCD35-24R
Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, resharpened
ea
QUOTE
UCD35-25
Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, new
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QUOTE
UCD35-25E
Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, exchanged
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QUOTE
UCD35-25R
Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, resharpened
ea
QUOTE
UCD35-30
Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, new
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QUOTE
UCD35-30E
Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, exchanged
ea
QUOTE
UCD35-30R
Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UCD35-35
Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, new
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QUOTE
UCD35-35E
Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, exchanged
ea
QUOTE
UCD35-35R
Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, resharpened
ea
QUOTE
UCD35-40
Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, new
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QUOTE
UCD35-40E
Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, exchanged
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QUOTE
UCD35-40R
Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, resharpened
ea
QUOTE
DiATOME CRYO DRY DIAMOND KNIVES, 45°
UCD45-15
Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, new
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QUOTE
UCD45-15E
Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, exchanged
ea
QUOTE
UCD45-15R
Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, resharpened
ea
QUOTE
UCD45-18
Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, new
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QUOTE
UCD45-18E
Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, exchanged
ea
QUOTE
UCD45-18R
Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, resharpened
ea
QUOTE
UCD45-21
Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, new
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QUOTE
UCD45-21E
Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, exchanged
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QUOTE
UCD45-21R
Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, resharpened
ea
QUOTE
UCD45-24
Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, new
ea
QUOTE
UCD45-24E
Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, exchanged
ea
QUOTE
UCD45-24R
Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, resharpened
ea
QUOTE
UCD45-25
Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, new
ea
QUOTE
UCD45-25E
Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, exchanged
ea
QUOTE
UCD45-25R
Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, resharpened
ea
QUOTE
UCD45-30
Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, new
ea
QUOTE
UCD45-30E
Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, exchanged
ea
QUOTE
UCD45-30R
Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UCD45-35
Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, new
ea
QUOTE
UCD45-35E
Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, exchanged
ea
QUOTE
UCD45-35R
Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, resharpened
ea
QUOTE
UCD45-40
Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, new
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QUOTE
UCD45-40E
Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, exchanged
ea
QUOTE
UCD45-40R
Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, resharpened
ea
QUOTE
DiATOME CRYO WET DIAMOND KNIVES, 35°
UCW35-15
Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, new
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QUOTE
UCW35-15E
Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, exchanged
ea
QUOTE
UCW35-15R
Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, resharpened
ea
QUOTE
UCW35-18
Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, new
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QUOTE
UCW35-18E
Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, exchanged
ea
QUOTE
UCW35-18R
Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, resharpened
ea
QUOTE
UCW35-21
Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, new
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QUOTE
UCW35-21E
Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, exchanged
ea
QUOTE
UCW35-21R
Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, resharpened
ea
QUOTE
UCW35-24
Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, new
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QUOTE
UCW35-24E
Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, exchanged
ea
QUOTE
UCW35-24R
Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, resharpened
ea
QUOTE
UCW35-25
Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, new
ea
QUOTE
UCW35-25E
Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, exchanged
ea
QUOTE
UCW35-25R
Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, resharpened
ea
QUOTE
UCW35-30
Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, new
ea
QUOTE
UCW35-30E
Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, exchanged
ea
QUOTE
UCW35-30R
Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, resharpened
ea
QUOTE
UCW35-35
Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, new
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QUOTE
UCW35-35E
Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, exchanged
ea
QUOTE
UCW35-35R
Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, resharpened
ea
QUOTE
UCW35-40
Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, new
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QUOTE
UCW35-40E
Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, exchanged
ea
QUOTE
UCW35-40R
Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, resharpened
ea
QUOTE
DiATOME CRYO WET DIAMOND KNIVES, 45°
UCW45-15
Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, new
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QUOTE
UCW45-15E
Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, exchanged
ea
QUOTE
UCW45-15R
Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, resharpened
ea
QUOTE
UCW45-18
Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, new
ea
QUOTE
UCW45-18E
Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, exchanged
ea
QUOTE
UCW45-18R
Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, resharpened
ea
QUOTE
UCW45-21
Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, new
ea
QUOTE
UCW45-21E
Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, exchanged
ea
QUOTE
UCW45-21R
Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, resharpened
ea
QUOTE
UCW45-24
Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, new
ea
QUOTE
UCW45-24E
Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, exchanged
ea
QUOTE
UCW45-24R
Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, resharpened
ea
QUOTE
UCW45-25
Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, new
ea
QUOTE
UCW45-25E
Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, exchanged
ea
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UCW45-25R
Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, resharpened
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UCW45-30
Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, new
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UCW45-30E
Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, exchanged
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UCW45-30R
Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, resharpened
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UCW45-35
Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, new
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UCW45-35E
Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, exchanged
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UCW45-35R
Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, resharpened
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UCW45-40
Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, new
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UCW45-40E
Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, exchanged
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UCW45-40R
Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, resharpened
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Histo and Histo Jumbo
Histo and Histo Jumbo
Histo Cryo Dry and Histo Cryo Wet
Histo Cryo Dry and Histo Cryo Wet
THE HISTO KNIFE FOR LIGHT MICROSCOPY
For Room Temperature
Knives are available with a 45° angle.
Section thickness range 0.2 - 10µm.
Cutting edge length 4, 6, and 8mm.
The knife is designed for the sectioning of hard and soft biological and industrial materials, non- embedded or embedded in methacrylate or epoxy resins.
The Histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.
For the production of our Histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.

Some of the advantages of our Histo diamond knives compared with glass knives:
  • Perfect sections, free of scores or compression
  • Serial sections without knife change
  • Thinner sections
  • Extreme durability of the cutting edge
  • No knife making procedure
  • Lifetime guarantee

Histo Jumbo knives
For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).
The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of the sample block increase the distinct advantages of our histo knives.

Features
  • Easy production of section ribbons (0.5-2µm)
  • No section loss
  • No folding
  • The same orientation of all sections
  • Easy collection of section ribbons
  • Multiple ribbons on one glass slide
  • Perfect for immuno-histo-chemistry


For Cryo Temperatures
Knives are available with a 45° angle.
Section thickness range 0.2 - 5ìm.
Cutting edge length 4 and 6mm.
The Histo Cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.

U3add6
The histology knife for light microscopy
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Histo Diamond knife
Histo Diamond knife
DiATOME HISTO KNIVES
UH45-40
Diamond knife, Histo, 45°, 4.0 - 4.9mm, new
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UH45-40E
Diamond knife, Histo, 45°, 4.0 - 4.9mm, exchanged
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UH45-40R
Diamond knife, Histo, 45°, 4.0 - 4.9mm, resharpened
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UH45-60
Diamond knife, Histo, 45°, 6.0 - 6.9mm, new
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UH45-60E
Diamond knife, Histo, 45°, 6.0 - 6.9mm, exchanged
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UH45-60R
Diamond knife, Histo, 45°, 6.0 - 6.9mm, resharpened
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UH45-80
Diamond knife, Histo, 45°, 8.0 - 8.9mm, new
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UH45-80E
Diamond knife, Histo, 45°, 8.0 - 8.9mm, exchanged
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UH45-80R
Diamond knife, Histo, 45°, 8.0 - 8.9mm, resharpened
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Histo Jumbo Diamond knife
Histo Jumbo Diamond knife
DiATOME HISTO JUMBO KNIVES
UHJ45-60
Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, new
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UHJ45-60E
Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, exchanged
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UHJ45-60R
Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, resharpened
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UHJ45-80
Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, new
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UHJ45-80E
Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, exchanged
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UHJ45-80R
Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, resharpened
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Histo Cryo Dry Diamond knife
Histo Cryo Dry Diamond knife
DiATOME HISTO CRYO DRY KNIVES
UHCD45-40
Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, new
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UHCD45-40R
Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, resharpened
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UHCD45-60
Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, new
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UHCD45-60R
Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, resharpened
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Histo Cryo Wet Diamond knife
Histo Cryo Wet Diamond knife
DiATOME HISTO CRYO WET KNIVES
UHCW45-40
Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, new
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UHCW45-40R
Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, resharpened
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UHCW45-60
Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, new
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UHCW45-60R
Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, resharpened
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Cryo Immuno
Cryo Immuno
DiATOME CRYO IMMUNO DIAMOND KNIVES

The cryo immuno knife guarantees the best possible sectioning for sucrose infiltrated samples

We recommend the cryo immuno knife also for sectioning frozen hydrated samples (CEMOVIS). The 35° angle is a good compromise between durability and cutting performance

The first cryo knife with a diamond platform guarantees the best possible sectioning for cryo-immuno-chemistry.
The diamond platform ensures easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters).
The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.

UCI35-20
Diamond knife, Cryo Immuno, 35°, 2.0mm, new
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UCI35-20R
Diamond knife, Cryo Immuno, 35°, 2.0mm, resharpened
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UCI35-30
Diamond knife, Cryo Immuno, 35°, 3.0mm, new
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UCI35-30R
Diamond knife, Cryo Immuno, 35°, 3.0mm, resharpened
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It is very important to remember that to successfully do dry cryosectioning you must eliminate electrostatic charges in the cryochamber. Our studies have shown that a single spike ioniser which produces a surplus of negative ions and is driven by high voltage is most effective in eliminating the electrostatic charges.
Please see our section on THE STATIC LINE II IONISER.

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Successfully dry cryosectioning
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Ultra Sonic Diamond knife
UUS35-30CU Ultra Sonic Diamond knife
DiATOME ULTRA SONIC DIAMOND KNIFE

The DiATOME ultra sonic, the oscillating diamond knife for room temperature ultramicrotomy, was developed in collaboration with Dr Daniel Studer, Lab. of Anatomy, University of Berne. A piezo actuator produces an oscillation of the knife at a desired frequency and amplitude, parallel with the cutting edge. A depression in the foot of the knife allows the oscillation parallel to the cutting edge. The depression is rigid in the north/south direction and guarantees stability in the cutting direction.
The knife produces ultrathin sections almost free of compression. The sections become thinner at the same thickness setting: since the volume of the section remains the same, the increased length leads to a decrease in thickness.

Specifications
Knife angle: 35°
Knife cutting range: 10-80nm
Knife cutting edge length: 3.0mm
Control Unit
Frequency: 25-45kHz, or automatic setting of the resonance
Amplitude: variable (Voltage 0-30V)
Mains voltage: 230V, 110V

UUS35-30
Diamond knife, Ultra Sonic, 35°, 3.0mm, new
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UUS35-30CU
Diamond knife, Ultra Sonic, 35°, 3.0mm, including control unit
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UUS35-30R
Diamond knife, Ultra Sonic, 35°, 3.0mm, resharpened
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Cryo AFM and Ultra AFM
Cryo AFM and Ultra AFM
DiATOME AFM DIAMOND KNIVES, CRYO AND ULTRA

Extremely smooth sample surfaces and best possible structure preservation.
The high quality DiATOME diamond knives are not just perfect for producing ultrathin and semi-thin sections, but also for surface sectioning of all kinds of biological and industrial specimens for AFM investigation.
Instead of a section as for TEM, the specimen surface is investigated using AFM. In order to achieve the best results for AFM investigation, only the highest quality diamond knives should be used.
Diatome Ultra AFM and Cryo AFM knives are specially tested to ensure that they meet the increased quality requirements of AFM investigations. They produce extremely smooth sample surfaces and guarantee the best possible structure preservation.
Available with a 35° angle and a cutting range of 10-100nm, cutting edge length 2mm and 3mm.

UCA35-20
Diamond knife, Cryo AFM, 35°, 2.0mm, new
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UCA35-20R
Diamond knife, Cryo AFM, 35°, 2.0mm, resharpened
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UCA35-30
Diamond knife, Cryo AFM, 35°, 3.0mm, new
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UCA35-30R
Diamond knife, Cryo AFM, 35°, 3.0mm, resharpened
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UUA35-20
Diamond knife, Ultra AFM, 35°, 2.0mm, new
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UUA35-20R
Diamond knife, Ultra AFM, 35°, 2.0mm, resharpened
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UUA35-30
Diamond knife, Ultra AFM, 35°, 3.0mm, new
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UUA35-30R
Diamond knife, Ultra AFM, 35°, 3.0mm, resharpened
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Diamond Knife Cleaning Sticks
UD060 Diamond Knife Cleaning Sticks
DIAMOND KNIFE CLEANING STICKS

High density polystyrene has been found most effective for cleaning of diamond knives. Instruction card is included.

UD060
Diamond knife cleaning sticks
AUD19.00
pk/4
ADD TO CART 
Static Line II ioniser
U500 Static Line II ioniser
Static Line II ioniser
Static Line II ioniser
STATIC LINE IONISER II
The Static Line II ioniser is an antistatic device which emits negative and positive ions, neutralising the electrostatic charging in the cryochamber.
The sections no longer stick to the knife edge, or bunch up one on top of the other, but float in a nice ribbon over the knife surface. The pickup of the sections can be done easily. The Static Line II ioniser is recommended for trimming, sectioning and section collection.
Range of applications
  • Sucrose protected specimens
  • Cell cultures in elatine/ sucrose
  • Frozen hydrated specimens
  • Dry cut Lowicryls at room temperature
  • Polymers and rubber


The Static Line II consists of a high voltage power pack, an ioniser electrode which is connected to the power pack by a cable, and a testing device, the MULTI CHECK.
A dial on the power pack allows for adjustment of the voltage. The correct ion emission can be achieved by turning the variable voltage dial.


Specifications
Voltage: 230 V, 115 V
Nominal frequency: 50-60Hz
Nominal output voltage: approx. 7-8 kV variable
Power consumption: approx. 50 VA
Short circuit output current: max. 3 mA
Dimensions: L173 x W113 x H110mm
Weight: approx. 3.5kg

U500
Static Line II ioniser
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Trimtools 20 and 45
Trimtools 20 and 45
Trimming procedure
Trimming procedure
DiATOME TRIM TOOLS
One trimming tool for all trimming needs, be it at room or cryo temperatures. The trimtool series supersedes the cryotrim and ultratrim lines of trimming tools. Resharpening is still available for these lines.
For successful ultramicrotomy in biology and materials science, precise trimming is mandatory.
The DiATOME trimming blades trimtool 45 and trimtool 20 will meet all trimming requirements, allowing quick, easy and accurate trimming at both room and cryo temperatures.
Due to the extreme sharpness of our diamond blades, less mechanical damage is applied to the sample during trimming. Very shiny sample faces and precise sides are the result.
The trimtool 45 produces pyramidal sides with an inclined angle of 45° and the trimtool 20 produces pyramidal sides with an inclined angle of 20°.

Area of application

Room temperature:
  • Epoxy or acrylate resin embedded biological samples
  • Metals
  • Polymers
  • Nanoparticles in polymers
  • Non-embedded brittle samples

Cryo temperatures:
  • Sucrose infiltrated and frozen hydrated biologoical samples
  • Soft polymers
UTT20
Trimtool 20, 20°, new
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UTT20-R
Trimtool 20, 20°, resharpened
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UTT45
Trimtool 45, 45°, new
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UTT45-R
Trimtool 45, 45°, resharpened
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U060 Heat Pen for ultramicrotomy
U060 Heat Pen for ultramicrotomy
U060R Heat Pen replacement tip
U060R Heat Pen replacement tip
HEAT PEN MAX WAX

For stretching of resin sections in ultramicrotomy and for making wax seals on glass knife troughs.
Its small, exposed heating element can reduce sectioning compression further than is possible using solvent vapour. It also reduces compression in semi-thin sections and eliminates the use of toxic chloroform vapour.
The element quickly forms wax seals around glass knives along the wax lined knife trough.
Two AA batteries power this model. The element is only heated when the switch is depressed; that is normally only for a couple of seconds, so batteries last a long time. In a very busy laboratory, these can be substituted with rechargeable batteries.
Replacement tips are available.

Documents

U060
Heat Pen for ultramicrotomy
AUD68.00
ea
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U060R
Heat pen replacement tip
10% DISCOUNT FOR 3
AUD18.00
ea
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